促性腺激素释放激素受体基因表达在青春期发育的雌性老鼠。

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Zapatero-Caballero H, Sanchez-Franco F, Fernandez-Mendez C, Garcia-San Frutos M, Botella-Cubells LM, Fernandez-Vazquez G

促性腺激素释放激素受体基因表达在青春期发育的雌性老鼠。

70年2月杂志。2004;(2):348 - 55。Epub 2003年10月15日。

PubMed ID

14561652 (在PubMed

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文摘

激性腺素释放素受体(GnRH-R)的适当表达gonadotrophs对促性腺激素信号至关重要,因此对促性腺激素分泌和性发展。在目前的工作中,我们研究了稳态GnRH-R mRNA水平的个体发生脑垂体后5天55岁的雌性老鼠,当达到性成熟。促性腺激素的发展变化单元(α,FSHbeta和LHbeta) mRNA水平也被评估。此外,角色的内源性促性腺成熟的变化GnRH-R和促性腺激素亚基基因表达研究。信使RNA水平由北部污点分析前脑垂体后的总RNA。含量最丰富的(5.0千碱基(kb)) GnRH-R mRNA增加缓慢从第五天到幼儿时期,将达到峰值,20天(约4倍增加比第五天)。此后GnRH-R mRNA的水平突然下降25天(下降75%和20天),然后略有下降,直到35天。平行变化观察4.5 kb GnRH-R基因的转录。α亚基mRNA在第五天容易检查出来的,其水平增加迅速通过婴儿时期的开始将达到峰值,第十天(3.2倍的增长与第五天);在35天就下降了85%。FSHbeta和LHbeta mRNA水平上升缓慢,直到天15 - 20,GnRH-R前很短的时间内。 Thereafter, the levels of both mRNAs fell until Day 35 (90% decrease vs. Day 15 for FSHbeta and 50% decrease vs. Day 20 for LHbeta). To ascertain whether developmental activation of the GnRH-R and gonadotropin subunit gene expression is GnRH dependent, we have studied the effect of blocking the endogenous GnRH action by treating developing female rats with the specific GnRH antagonist cetrorelix (1.5 mg/kg body weight/wk, s.c.) through the infantile (Days 5-20) and the juvenile period (Days 20-35). Cetrorelix completely blocked the rise of levels of the two most abundant species, 5.0 kb and 4.5 kb, of GnRH-R mRNA during the infantile phase and dropped them to almost undetectable levels during the juvenile prepubertal period. Cetrorelix also abolished the developmental rise of gonadotropin beta subunit mRNAs during the two periods of the study. In contrast, alpha subunit gene expression tended to decrease, but not significantly, with cetrorelix treatment during the two periods. These data demonstrate that sexual maturation of female rats is advanced by an early and strong induction of GnRH-R and gonadotropin subunit gene expression during the infantile period, followed by weaker persistent activation during puberty. Developmental GnRH-R and gonadotropin beta subunit gene expression is almost entirely GnRH dependent, not only in the juvenile prepubertal stage but also during the infantile period.

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药物靶点

药物	目标	类	生物	药理作用	行动
Cetrorelix	促性腺激素释放激素受体	蛋白质	人类	是的	拮抗剂	细节